HAPLOPHYLLUM TUBERCULATUM PDF

Ethanolic extract of the aerial parts of Haplophyllum tuberculatum demonstrated an efficient anti-fungal activity against Aspergillus fumigates, Geotricum. Phytochemistry. Jun;57(4) Alkaloids from Haplophyllum tuberculatum. Al-Rehaily AJ(1), Al-Howiriny TA, Ahmad MS, Al-Yahya MA, El- Feraly FS. Description & Remarks. Development of adventitious shoots have been observed on exposed roots in a small wadi near Kibbutz Samar. See more from the.

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January 09, ; Accepted date: February 16, ; Published date: J Pharmacogn Nat Prod 2: This is an open-access article distributed under the terms of the Creative Commons Fuberculatum License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Ethanolic extract of the aerial parts of Haplophyllum tuberculatum demonstrated an efficient anti-fungal activity against Aspergillus fumigates, Haplopgyllum candidum haplophyllu Syncephalastrum racemosum with MIC 0.

Volatile oil of the aerial parts demonstrated significant antibacterial effect against Enterococcus faecalis and Lactobacillus acidophilus MIC 1. The essential oils from aerial parts and flowers exhibited a remarkable acute anti-inflammatory activity against carrageenan induced oedema in rats 9.

About 70 species of the genus Haplophyllum are present in the Mediterranean region [ 1 ]. In Egypt, the flowering aerial parts are used as a decoction for rheumatic pains [ 2 ]. In Oman, the leaves are used for relieving arthritis and also used for treatment of skin infections [ 3 ]. The plant is well known for its richness in alkaloids, fixed oils, volatile oils and furanocoumarins [ 45 ].

The ethanol extract of H. No published report concerning the potential biological activities of the volatile oil of the aerial and the flower of Libyan H. The aim of the study is to screen certain biological activities of the ethanolic extract of the aerial parts and essential oils of Libyan H.

Samples of the aerial parts and flowers of Haplophyllum tuberculatum were obtained from Benghazi, Libya. Collected samples were identified by Dr.

A voucher specimen of the aerial parts and the flowers of H. Fresh samples of aerial parts and flowers of H. The essential oils were dried over anhydrous sodium sulfate tuberculatuk kept refrigerated. The air-dried powdered aerial parts of H. The ethanol extract was evaporated under reduced pressure to give 20 g greenish brown semi-solid residue.

The antimicrobial activity was performed against selected eight bacterial and four fungal strains of standard properties. These haaplophyllum maintained in the regional center for mycology and biotechnology, Al Azhar University.

Ampicillin, gentamycin, and vancomycin were used as a positive control for bacterial strain antibacterial standards, Sedico Pharmaceutical Co. The plates were done in triplicate. Results of the anti-microbial testing of the ethanol extract and essential oils of H.

Varying concentrations of the samples A tube containing broth media only was seeded with the test organisms to serve as control. The tubes were then examined for growth by observing for turbidity [ 10 ]. All animals were kept on standard laboratory diet under hygienic conditions.

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Haplophyllum tuberculatum (Forssk.)

Water was supplied ad libitum. Determination of median lethal dose LD The LD 50 of the volatile oils was determined according to the procedures developed by Karber [ 11 ]. It was determined according to the rat paw oedema methods [ 12 ].

Five groups of male albino rats were used 6 tuuberculatum each.

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The first group received 1 ml saline orally negative control. The second group was given indomethacin orally positive control. The other groups received the tested samples in the dose given in Table 3. Drugs were orally administered 1 hr prior to carrageenan injection.

Oedema was induced in the rat right hind paw by S. Thickness of the right hind paw mm was measured immediately before and 1, 2, 3 and 4 hr post carrageenan injection with a micrometer caliber. Both paws were excised and weighed separately using an electric balance.

Effect of the ethanol extracts of H. The anti-oxidant activity was calculated by the determination of glutathione in blood of alloxan-induced diabetic rats adopting the methods of Beutler et al. The animals were divided into 5 groups 6 animals each.

A group of diabetic rats was kept non-treated, another group received daily the reference drug Vitamin E and the other groups received the tested samples of H. Blood samples were taken after a week for the determination of glutathione. The results obtained were recorded in Table 4. Anti-oxidant activity of the ethanol extracts and essential oils of H. A probability value of less than 0. Hydrodistillation of the aerial tuberxulatum and flowers of H.

The haplophyplum yield of the hydrodistillation of the flowers was about 3 times in the aerial parts. The specific gravity and refractive index were 0. When screened for tubedculatum activity, the essential oil of the aerial parts AO and flowers FO of H.

This means that they were resistant to our samples or that they necessitated the use of higher concentrations as compared to the standard antibacterial drug Gentamicin Table 1.

The essential oil of the flowers showed lower inhibition zones than the essential oil of the aerial parts compared to standard antibiotics Table 1. The most sensitive microorganism is Escherichia coli its sensitivity ranged from Escherichia coli were inhibited by the essential oil of the aerial parts with MICs 3.

However, the essential oil of the aerial parts displayed a remarkable growth inhibitory effect against the fungus Aspragillus fumigates The MIC of the essential oil of the aerial parts for the tested Aspergillus fumigates, Syncephalastrum racemosum and Geotricum candidum was 0. The antibacterial effect of the oil of the aerial parts may be attributed to its monoterpenes constituents. The antibacterial mechanism of oxygenated monoterpenes against E.

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The volatile oils were slightly more effective against Gram positive than Gram negative bacteria. This is in agreement with observations made by other authors that Gram positive bacteria were more susceptible to essential oils than Gram negative ones [ 16 ].

Results showed that the essential oil of the aerial parts was much more active against the twelve tested microorganisms than the essential oil of the flowers.

The essential oil of the aerial parts inhibit growth of Haplophylum tuberculosis and Methicillin-resistant Staphylococcus aureus The hours LD 50 was approximately more than 0. These results huberculatum that the essential oils and the ethanolic extract are safe and non-toxic.

The essential oil of the aerial parts of H. Percentage of oedema inhibition of the essential oil of the aerial parts, the essential oil of the flowers and the ethanolic extract of the aerial parts were 9. Recorded results Table 4 revealed also that the essential oils exerted a remarkable anti-oxidant activity. To the best of our knowledge, this is the first report on the potential biological activities of the alcoholic extract and essential oil of Libyan H.

Anti-microbial activity of essential oil is difficult to correlate to a specific compound due to their complexity and variability and in general, is attributed to phenolic and hydroxyl groups.

Alkaloids from Haplophyllum tuberculatum.

Although other active terpenes, alcohols, aldehydes and esters can contribute to the overall anti-microbial effect of essential oils [ 17 ]. The mechanism of action of essential oils against bacteria has now been partly elucidated. Prior to the availability of data, assumptions about its mechanism of action were made on the basis of its hydrocarbon structure and lipophilicity.

Since hydrocarbons partition preferentially enter into biological membranes and disrupt their vital functions [ 18 ]. Most of the anti-microbial activity in essential oils is found in the oxygenated terpenoids e. Accepted mechanisms of anti-microbial interaction that produce synergism include the sequential inhibition of a common biochemical pathway, inhibition of protective enzymes and use of cell wall active agents to enhance the uptake of other anti-microbials [ 22 ].

Terpinenol modulates the vasodilation and plasma extravasation associated with histamine-induced inflammation in humans [ 24 ].

Haplophyllum tuberculatum (Forssk.) | Flora of Israel Online

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