ELUTRAP MANUAL PDF
The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Samples are purified with . azim58 – Official elutrap documentation. Elutrap protocol. “C:\kurt\storage\CIM Research Folder\DR\\\ELUTRAP\ “. The Elutrap, seen from above, is placed on a horizontal agarose gel electrophoresis chamber. Outer limits of the trap are defined with disposable membranes at.
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Cut out an adequate piece of dialysis membrane and place in the beaker containing the DI water. Then, run the unit at 50V overnight for the final collection. The advantages of this system over previously used techniques such as diffusion and simple electro-elution are a high recovery rate, b high reproducibility, c high purity elutral recovered material and d the ease of use 1. Leakage can be detected by the appearance of water eluttap the paper filter. Electrophoresis chamber with a central 4-channel tray insert.
Help us improve your experience by sending an error report. Our site is powered by industry leading security standards for your protection. The manhal placement is adjustable, allowing final elution trap volumes to be optimized for the particular assay. Replacement electrophoresis chamber for the Elutrap Electroelution system.
Whatman Elutrap Electroelution System, Whatman 10404090 Replacement Components BT1 Membranes
Conclusion Organic solvents may damage the device and should be removed promptly. If you cannot enable cookies in your browser, please contact us — we are always here to help! Always keep the BT1 and manuzl dialysis membrane moist.
Place the dialysis membrane between the screw and the closed U insert in the large chamber of the device. Take the BT1 membrane out using forceps and clean thoroughly with DI water to remove all traces elutrpa glycerol. It is very important to elturap the sloping surface of the BT1 membrane next to the triangular mark on the side of the device. Hence, two filters can be prepared from one piece of the dialysis membrane.
The OD of subsequent collections should be lower as the concentration of the sample gets diluted with the buffer. Take care not to damage the membranes during collection. The Elutrap system can also be used for concentration of dilute solutions.
Elutrap Electrophoresis Chamber
The notched surface of the inserts always faces the central elution chamber containing the gel pieces. Web browser based cookies allow us to customize our site for you, save items in your cart, and provide you with a great experience when shopping LabPlanet. Please change the country on your profile in order to switch to another country store.
Please add ” labplanet. Remember to cut the dialysis membrane with one edge slightly higher than the other similar to the shape of the BT1 membrane. Elutrap Electro-Elution System Introduction Elutrap electro-elution system is a membrane trap elution system used for the extraction, concentration and dialysis of DNA and other charged molecules above the size of 5 kDa 1.
The Elutrap system is designed to isolate nucleic acids and proteins from agarose or polyacrylamide gel slices by electroelution. Make sure that the inserts are tightly placed and rest on the designated slot. Also make sure that the gel pieces are completely covered with the buffer.
Info for a Biotech Student: Elutrap electro-elution system
Dialysis membrane — one per each device used. You can also explore other items in the Electrophoresis system power suppliesOther Lab ApparatusLab Apparatus categories yourself to try and find the perfect replacement for you! The buffer in the electrophoresis chamber should cover the electrodes. We are an authorized supplier to all federal and state government agencies, as well as to research and educational institutions, and can facilitate volume orders on many types of laboratory equipment, lab consumables, and other products to help fulfill your specific needs.
The same applies to handling of the equipment. Do not stack gel slices above the device height. Collect using a loading tip, moving the tip carefully along the edges to recover the entire sample. Following completion of the electro-elution, proceed with ethanol precipitation. Assembly of the Elutrap system is easy. It is essential to then autoclave the buffer to remove all traces of DEPC.
Loosen the screws of the elutrap device. To leave a review Register or Log In. About Us Knowledge Center Policies. However, take precautions to keep the BT2 membrane dry for as long as possible.